| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 451 | Bacillus subtilis | AGCCATCGTCACGCGTTT | AGAAGCGACGGGTGAATGT | 60.36 | 59.33 | 291 | 45 |
100.00%
|
100.00%
|
| Primer 452 | Bacillus subtilis | GCGGTGGCATTGGCAAAA | GCGGACAGGTCAGCTTGTA | 59.97 | 59.71 | 160 | 45 |
100.00%
|
100.00%
|
| Primer 453 | Bacillus subtilis | AAACGTGGCACCGCATTT | TGCGACATACAGCAGCACA | 58.88 | 60.01 | 277 | 45 |
100.00%
|
50.00%
|
| Primer 454 | Bacillus subtilis | CGAGAATGCAGCCAGCTCT | TCAGCAGCGCATCGACTT | 60.15 | 59.74 | 196 | 44 |
100.00%
|
100.00%
|
| Primer 455 | Bacillus subtilis | TTGCAAACACGCCAGCAG | GCCCATGTGAAGGTACGCA | 59.59 | 60.38 | 179 | 44 |
100.00%
|
50.00%
|
| Primer 456 | Bacillus subtilis | CTTGCAAACACGCCAGCA | GCCCATGTGAAGGTACGCA | 59.59 | 60.38 | 180 | 44 |
100.00%
|
50.00%
|
| Primer 457 | Bacillus subtilis | TGCGCACGGAGGCATTTA | AGCTCGGGTTCAGCAACA | 60.05 | 59.17 | 220 | 44 |
100.00%
|
100.00%
|
| Primer 458 | Bacillus subtilis | TGCGCACGGAGGCATTTA | CTTCAGCTCGGGTTCAGCA | 60.05 | 60.00 | 224 | 44 |
100.00%
|
100.00%
|
| Primer 459 | Bacillus subtilis | TGCGCACGGAGGCATTTA | TCAGCTCGGGTTCAGCAA | 60.05 | 58.85 | 222 | 44 |
100.00%
|
100.00%
|
| Primer 460 | Bacillus subtilis | TGCGCACGGAGGCATTTA | TTCAGCTCGGGTTCAGCA | 60.05 | 58.85 | 223 | 44 |
100.00%
|
100.00%
|